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CytoCell fluorescence in situ hybridisation (FISH) logo.

Product summary

  • Chromosomes
    1314152122
  • Labels  
  • Disease focus Constitutional
  • Regulatory status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.
Cytocell Catalogue Probe Packaging With Amber Tube
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Overview Product documentation Customer feedback

Overview

The Nucleolar Organiser Regions (NOR) contain clusters of genes which code for the three largest structural rRNA molecules (5.8S, 18S and 28S).

These rRNA genes are critically important for the viability of the cell and represent around 0.5% of the human diploid genome. They are found in the short arm of the acrocentric chromosomes and are the region around which the nucleoli develop at the end of mitosis1.

A NOR can translocate to a terminal region of another chromosome. In rare instances this can be pathogenic, particularly when the translocation has led to a deletion at the tip of the recipient chromosome2. It has been suggested that the restructuring of the rRNA genes is the most common chromosomal alteration in adult solid tumours3.

In routine cytogenetic analysis, NOR can be used to delineate marker chromosomes through a process of silver staining the NOR (known as AgNOR staining4). However, the technique relies on translation of protein and if this is not present, conventional silver staining will not stain the NOR. Cytocell's FISH probe has been developed to overcome this problem so that presence of the acrocentric chromosomes in the marker can be detected.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell Acro-P-Arm probe.

Microscope image

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Product documentation

What our customers say about CytoCell probes...

Our lab has been using a wide range of CytoCell FISH probes for a number of years, and have been increasing this range all the time. The probes have clear bright signals and show good reproducibility. CytoCell provides fast delivery of catalogue probes, and are very responsive when we have any queries or problems with their products.

Bridget Manasse, Addenbrooke's Hospital, Cambridge University Hospitals NHS Foundation Trust, UK.

Bridget Manasse

Addenbrookes Hospital, Cambridge University Hosiptals NHS Foundation Trust, UK

In our hands, CytoCell FISH probes have proven to be of the highest quality with bright, easy to interpret signals, thus providing confidence in our results. OGT's customer support is outstanding, as their staff are extremely knowledgeable and truly care about their customers and their customers’ needs.

Jennie Thurston, Director of Cytogenetics, Carolinas Pathology Group, USA.

Jennie Thurston

Director of Cytogenetics, Carolinas Pathology Group, USA

I first came across CytoCell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing CytoCell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time.

Elizabeth Benner, Medical Technologist, University of Arizona Health Network, USA.

Elizabeth Benner

Medical Technologist, University of Arizona Health Network, USA

We have been working with CytoCell fish probes for two decades because of their excellent clarity and intensity regardless of the size of the probe. It is so clear and simple to detect.

Dr. Marina Djurisic

Head of Laboratory of Medical Genetics, Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic”, Serbia

The quality and consistency of CytoCell’s probes means I can trust the results, and my clients get their results in a timely manner.

Dr. Theresa C. Brown, Director, Cytogenetics Laboratory, Hayward Genetics Center, Tulane University School of Medicine, USA.

Dr. Theresa C. Brown

Director, Cytogenetics Laboratory, Hayward Genetics Center, Tulane University School of Medicine, USA

It was very important for us to have more consistent results with our probes — easy-to-read bright signals and a range of vial sizes, which is much more cost-effective.

Janet Cowan, Director of the Cytogenetics Laboratory, Tufts Medical Center, USA.

Janet Cowan, PhD

Director of the Cytogenetics Laboratory, Tufts Medical Center, USA

Not only do CytoCell offer an extensive range of high-quality FISH probes, the customer support is also excellent — providing fast access to all the probes I need. The probes are highly consistent with bright signals allowing easy scoring of results.

Dr. Eric Crawford

Senior Director, Genetics Associates Inc., USA

The quality and reproducibility of results using the CytoCell kit has been vital in accurately detecting co-deletions in our glioma investigations. We now have a cost-effective test that we can rely on that is also easy to use and interpret. We've been consistently impressed with this kit - not to mention the support offered by OGT's customer service, and have completely transitioned over to CytoCell probes.

Gavin Cuthbert, FRCPath

Head of Cancer Cytogenetics, Northern Genetics Servce, Newcastle, UK

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References

  1. McClintock B, Z Zellforsch Mikrosk Anat 1934;21:294-328
  2. Gardner RJM, Grant R Sutherland, Chromosome abnormalities and genetic counseling, Oxford University Press p240 2004
  3. Stults DM et al., Cancer Res 2009;69(23):9096-104
  4. Goodpasture C, Bloom SE, Chromosoma 1975;53:37-50