Tips include advice for using spectrophotometers and what blanking buffer you should use.
Quantitate your gDNA using a Nanodrop spectrophotometer or a fluorometer e.g. Qubit.
Pete Gray
Array and NGS Field Application Specialist, Europe
Spectrophotometers can over estimate the amount of DNA. For lower concentrations, use a fluorescent assay in addition to spectrophotometer (e.g. Qubit).
Mike Ornelas
FAS Manager – North America
Ensure you use the correct blanking buffer with the spectrophotometer. The blanking buffer should be the same as the buffer that the DNA sample is dissolved in.
Pete Gray
Array and NGS Field Application Specialist, Europe
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