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Universal NGS Complete Workflow Solution protocol tutorials

Part 1 NGS Workflow – DNA Fragmentation, End Repair, And 3’ End A Tailing Play the video.

NGS workflow – DNA fragmentation, end repair, and 3’ end A-tailing

Part I – Video illustrates the DNA fragmentation, end repair, and 3’ end A-tailing process in the Universal NGS Complete Workflow Solution.

Part 2 NGS Workflow – Adapter Ligation Play the video.

NGS workflow – Adapter ligation

Part II - Video illustrates the adapter ligation process and the purification of the ligated library in the Universal NGS Complete Workflow Solution.

Part 3 NGS Workflow – Pre Capture PCR Play the video.

NGS workflow – Pre-capture PCR

Part III - Video illustrates the pre-capture PCR of the ligated library and its purification in the Universal NGS Complete Workflow Solution.

Part 4 NGS Workflow – Hybridisation Play the video.

NGS workflow – Hybridisation

Part IV - Video illustrates the hybridisation process with design specific baits in the Universal NGS Complete Workflow Solution.

Part 5 NGS Workflow Post Capture PCR, Sample Pooling, And Sequencing Play the video.

NGS workflow - Post-capture PCR, sample pooling, and sequencing

Part V – Video illustrates the post-capture PCR of the hybridised samples, and the final pooling for sequencing in the Universal NGS Complete Workflow Solution.

NGS library preparation protocol tutorials for solid tumour samples

1. DNA Fragmentation Play the video.

NGS library preparation protocol for solid tumour samples - DNA fragmentation

This video illustrates the key steps for enzymatic DNA fragmentation prior to library preparation using the SureSeq™ NGS Library Preparation Kit for solid tumour samples.

2. Ampure Beads Play the video.

NGS library preparation protocol for solid tumour samples - AMPure beads

This video illustrates the key steps for AMPure® purification using the SureSeq™ NGS Library Preparation Kit for solid tumour samples.

3. Magnetic Beads Play the video.

NGS library preparation protocol for solid tumour samples - Preparation of magnetic beads

This video illustrates the key steps for Dynabeads™ M270 Streptavidin magnetic bead preparation using the SureSeq™ NGS Library Preparation Kit for solid tumour samples.

4. Hybrid Capture Play the video.

NGS library preparation protocol for solid tumour samples – Hybrid capture

This video illustrates the key steps for hybrid capture using the SureSeq™ NGS Library Preparation Kit for solid tumour samples.

5. Wash Streptavidin Beads Play the video.

NGS library preparation protocol for solid tumour samples – Wash streptavidin beads

This video illustrates the key steps for post-hybridisation bead washing using the SureSeq™ NGS Library Preparation Kit for solid tumour samples.

Interpret NGS Analysis Software tutorials

1. Introduction To Interpret NGS Play the video.

Introduction to Interpret NGS analysis software

Learn about Interpret, OGT’s powerful and easy-to-use next generation sequencing analysis solution, designed to work seamlessly with SureSeq and CytoSure NGS panels.

2. FASTQ Upload Play the video.

Interpret NGS analysis software - FASTQ upload

Learn how easy it is to upload sample FASTQ files into OGT’s Interpret software and use them to create a batch for data analysis.

3. Viewing Analysis Batches Play the video.

Interpret NGS analysis software - Viewing analysis batches

Learn how simply you can navigate round your analysis batch results and generate batch reports up front of variant analysis.

4. QC Metrics Play the video.

Interpret NGS analysis software – QC metrics

Learn how Interpret software creates easy to navigate displays of QC metrics to help users access the performance of their sequencing runs.

5. Analysis Results By Variants Play the video.

Interpret NGS analysis software – Analysis results by variants

Find out how to view analysis results by variants and explore using this feature in the Interpret software.

6. Variant results v2 Play the video.

Interpret NGS analysis software – Variant results

Discover how to navigate and modify the Variant results page, selecting aberration types and customising data analysis displays and tables with the many options available.

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